Summary
Type: SPE
Application #: 305730
Catalog#: 730070 - SPE Cartridges, Chromabond, Silica, 6ml, PP, 500mg, 30/pk
Detection:
Conditions: Column conditioning (recommended): 6 mL hexane
(Do not let run the column dry.)
Sample aspiration: 200 µL hexane extract was loaded onto the column
Elution 1: 10 mL hexane (HC fraction)
Elution 2: 4 mL hexane – chloroform (1:1, v/v) (non-HC fraction)
Elution 3: 5 mL chloroform (polar non-HC fraction)
Elution 4: 5 mL methanol (more polar non-HC fraction)
Authors: Dietemann et al.
Source: PNAS, September 2, 2003, vol. 100, no. 18, 10341–1